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Biochemical and Molecular Responses Underlying the Contrasting Phosphorus Use Efficiency in Ryegrass Cultivars.

ABSTRACT: Improving plant ability to acquire and efficiently utilize phosphorus (P) is a promising approach for developing sustainable pasture production. This study aimed to identify ryegrass cultivars with contrasting P use efficiency, and to assess their associated biochemical and molecular responses. Nine ryegrass cultivars were hydroponically grown under optimal (0.1 mM) or P-deficient (0.01 mM) conditions, and P uptake, dry biomass, phosphorus acquisition efficiency (PAE) and phosphorus utilization efficiency (PUE) were evaluated. Accordingly, two cultivars with high PAE but low PUE (Ansa and Stellar), and two cultivars with low PAE and high PUE (24Seven and Extreme) were selected to analyze the activity and gene expression of acid phosphatases (APases), as well as the transcript levels of P transporters. Our results showed that ryegrass cultivars with high PAE were mainly influenced by root-related responses, including the expression of genes codifying for the P transporter LpPHT1;4, purple acid phosphatase LpPAP1 and APase activity. Moreover, the traits that contributed greatly to enhanced PUE were the expression of LpPHT1;1/4 and LpPHO1;2, and the APase activity in shoots. These outcomes could be useful to evaluate and develop cultivars with high P-use efficiency, thus contributing to improve the management of P in grassland systems.

SUBMITTER: Pontigo S

PROVIDER: S-EPMC10057710 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature

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Biochemical and Molecular Responses Underlying the Contrasting Phosphorus Use Efficiency in Ryegrass Cultivars.

Pontigo Sofía S Parra-Almuna Leyla L Luengo-Escobar Ana A Poblete-Grant Patricia P Nunes-Nesi Adriano A Mora María de la Luz ML Cartes Paula P

Plants (Basel, Switzerland) 20230308 6

Improving plant ability to acquire and efficiently utilize phosphorus (P) is a promising approach for developing sustainable pasture production. This study aimed to identify ryegrass cultivars with contrasting P use efficiency, and to assess their associated biochemical and molecular responses. Nine ryegrass cultivars were hydroponically grown under optimal (0.1 mM) or P-deficient (0.01 mM) conditions, and P uptake, dry biomass, phosphorus acquisition efficiency (PAE) and phosphorus utilization ...[more]

PMID: 36986913

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Project description:Background and aimsPhosphate (Pi) is one of the most limiting nutrients for agricultural production in Brazilian soils due to low soil Pi concentrations and rapid fixation of fertilizer Pi by adsorption to oxidic minerals and/or precipitation by iron and aluminum ions. The objectives of this study were to quantify phosphorus (P) uptake and use efficiency in cultivars of the species Coffea arabica L. and Coffea canephora L., and group them in terms of efficiency and response to Pi availability.MethodsPlants of 21 cultivars of C. arabica and four cultivars of C. canephora were grown under contrasting soil Pi availabilities. Biomass accumulation, tissue P concentration and accumulation and efficiency indices for P use were measured.Key resultsCoffee plant growth was significantly reduced under low Pi availability, and P concentration was higher in cultivars of C. canephora. The young leaves accumulated more P than any other tissue. The cultivars of C. canephora had a higher root/shoot ratio and were significantly more efficient in P uptake, while the cultivars of C. arabica were more efficient in P utilization. Agronomic P use efficiency varied among coffee cultivars and E16 Shoa, E22 Sidamo, Iêmen and Acaiá cultivars were classified as the most efficient and responsive to Pi supply. A positive correlation between P uptake efficiency and root to shoot ratio was observed across all cultivars at low Pi supply. These data identify Coffea genotypes better adapted to low soil Pi availabilities, and the traits that contribute to improved P uptake and use efficiency. These data could be used to select current genotypes with improved P uptake or utilization efficiencies for use on soils with low Pi availability and also provide potential breeding material and targets for breeding new cultivars better adapted to the low Pi status of Brazilian soils. This could ultimately reduce the use of Pi fertilizers in tropical soils, and contribute to more sustainable coffee production.

Project description:BackgroundPlants utilize a variety of mechanisms to adapt to fluctuations in phosphorus (P) availability. Potatoes, in comparison to other crops, often display reduced phosphorus use efficiency (PUE) due to their underdeveloped root systems; therefore, understanding the mechanisms underlying PUE is critical for improving it. This study aimed to evaluate the morphological and physiological responses of potatoes to different P levels, with a focus on root system alterations and PUE. Two potato cultivars, a table potato (cv. Milva) and a starch potato (cv. Lady Claire), were subjected to varying P levels (0.5, 2, 5, and 30 mg P L-1 supplied as KH2PO4) in a hydroponic system. Additionally, the plants grown under 0.5 and 2 mg P L-1 were treated with plant growth-promoting Bacillus subtilis (B. subtilis), compared to untreated controls, to investigate the effectiveness of B. subtilis in addressing P deficiency. B. subtilis inoculation was performed by adding a bacterial suspension weekly to the hydroponic nutrient solution.ResultsThe findings illustrated Milva's ability to efficiently allocate P and sugars to its roots under low P levels, thereby enhancing biomass and facilitating increased P uptake and PUE. Conversely, Lady Claire exhibited lower P assimilation efficiency under low P levels but demonstrated improved efficiency under high P availability. The concentration of P in the nutrient solution affected P uptake and several factors believed to be involved in P utilization, such as root morphology, sugar and indole-3-acetic acid concentration in the roots, and acid phosphatase activity. Gene expression analyses underscored the pivotal roles of StPHT1;1 and StPHT2;1 in P translocation to shoots, particularly in Lady Claire. Inoculation with B. subtilis improved P acquisition efficiency by 10% under low phosphorus levels (P0.5 and P2), particularly in Lady Claire, where shoot and root phosphorus contents increased by 13-25% and 4-13%, respectively. Additionally, B. subtilis displayed higher efficacy in mitigating P deficiency in Lady Claire compared to Milva, particularly under low P levels (P0.5 and P2).ConclusionMilva showed greater phosphorus efficiency than Lady Claire under low P conditions, attributed to higher P and sugar levels in roots, enhancing root growth, P uptake, and translocation to shoots, particularly to young leaves. However, Lady Claire demonstrated a notable increase in P uptake and enhanced responsiveness to B. subtilis inoculation, particularly under low P levels (P0.5 and P2). These findings provide valuable insights for optimizing P management strategies to improve PUE in potatoes, especially under low P levels.

Dataset Information

Biochemical and Molecular Responses Underlying the Contrasting Phosphorus Use Efficiency in Ryegrass Cultivars.

Publications

Biochemical and Molecular Responses Underlying the Contrasting Phosphorus Use Efficiency in Ryegrass Cultivars.

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