The prophage-encoded transcriptional regulator AppY has pleiotropic effects on E. coli physiology.

ABSTRACT: Bacterial genome diversity is influenced by prophages, which are viral genomes integrated into the bacterial chromosome. Most prophage genes are silent but those that are expressed can provide unexpected properties to their host. Using as a model E. coli K-12 that carries 9 defective prophages in its genome, we aimed at highlighting the impact of genes encoded by prophages on host physiology. We focused our work on AppY, a transcriptional regulator encoded on the DLP12 prophage. By performing RNA-Seq experiments, we showed that AppY production modulates the expression of more than 200 genes. Among them, 11 were identified by ChIP-Seq as direct AppY targets. AppY directly and positively regulates several genes involved in the acid stress response including the master regulator gene gadE but

SUBMITTER: Derdouri N 

PROVIDER: S-EPMC10057817 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature