Analysis of Essential Isoprene Metabolic Pathway Proteins in Variovorax sp. Strain WS11.

ABSTRACT: Isoprene monooxygenase (IsoMO, encoded by isoABCDEF) initiates the oxidation of the climate-active gas isoprene, with the genes isoGHIJ and aldH nearly always found adjacent to isoABCDEF in extant and metagenome-derived isoprene degraders. The roles of isoGHIJ and aldH are uncertain, although each is essential to isoprene degradation. We report here the characterization of these proteins from two model isoprene degraders, Rhodococcus sp. strain AD45 and Variovorax sp. strain WS11. The genes isoHIJ and aldH from Variovorax and aldH from Rhodococcus were expressed individually in Escherichia coli as maltose binding protein fusions to overcome issues of insolubility. The activity of two glutathione S

SUBMITTER: Rix GD 

PROVIDER: S-EPMC10057887 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature