Effect of Hydrogen Sulfide on Essential Functions of Polymorphonuclear Leukocytes.

ABSTRACT: Impaired polymorphonuclear leukocyte (PMNL) functions contribute to increased infections and cardiovascular diseases in chronic kidney disease (CKD). Uremic toxins reduce hydrogen sulfide (H₂S) levels and the anti-oxidant and anti-inflammatory effects of H₂S. Its biosynthesis occurs as a side process of transsulfuration and in the disposal of adenosylhomocysteine, a transmethylation inhibitor and proposed uremic toxin. PMNL chemotaxis was measured by the under-agarose method, phagocytosis, and oxidative burst by flow cytometry in whole blood and apoptosis by determining DNA content by flow cytometry and morphological features by fluorescence microscopy. Sodium hydrogen sulfide (NaHS), diallyl trisulphide (DATS) and diallyl disulphide (DADS), cysteine, and GYY4137 were

SUBMITTER: Farahat S 

PROVIDER: S-EPMC10058000 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature