Chromatin Remodeling via Retinoic Acid Action during Murine Spermatogonial Development.

ABSTRACT: Spermatogonial differentiation is a process that commits germ cells to the complex process of spermatogenesis. Spermatogonial differentiation is mediated by the action of retinoic acid, which triggers major morphological and transcriptional changes. While these transcriptional changes have been well explored, there has been little effort devoted to epigenetic regulation surrounding spermatogonial development. This study aimed to uncover the timing and dynamics of chromatin organization during spermatogonial development within the context of these transcriptional changes. Using germ cell synchrony and the assay for transposase accessible chromatin and next generation sequencing (ATAC-seq) to isolate subpopulations of developing spermatogonia and identify accessible regions within their geno

SUBMITTER: Schleif C 

PROVIDER: S-EPMC10058303 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature