High-Throughput Gel Microbeads as Incubators for Bacterial Competition Study.

ABSTRACT: Bacteria primarily live in structured environments, such as colonies and biofilms, attached to surfaces or growing within soft tissues. They are engaged in local competitive and cooperative interactions impacting our health and well-being, for example, by affecting population-level drug resistance. Our knowledge of bacterial competition and cooperation within soft matrices is incomplete, partly because we lack high-throughput tools to quantitatively study their interactions. Here, we introduce a method to generate a large amount of agarose microbeads that mimic the natural culture conditions experienced by bacteria to co-encapsulate two strains of fluorescence-labeled Escherichia coli. Focusing specifically on low bacterial inoculum (1-100 cells/capsule), we demonstrate a study on t

SUBMITTER: Nguyen-Le TA 

PROVIDER: S-EPMC10058504 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature