Plasmodium vivax MSP1-42 kD Variant Proteins Detected Naturally Induced IgG Antibodies in Patients Regardless of the Infecting Parasite Phenotype in Mesoamerica.

ABSTRACT:

Background

The serological tests using blood stage antigens might be helpful for detecting recent exposure to Plasmodium parasites, and seroepidemiological studies would aid in the elimination of malaria. This work produced recombinant proteins of PvMSP1₄₂ variants and evaluated their capacity to detect IgG antibodies in symptomatic patients from Mesoamerica.

Methods

Three variant Pvmsp1₄₂ genes were cloned in the pHL-sec plasmid, expressed in the Expi293F™ eukaryotic system, and the recombinant proteins were purified by affinity chromatography. Using an ELISA, 174 plasma or eluted samples from patients infected with different P. vivax haplotypes were evaluated against PvMSP1₄₂ proteins and to a native blood stage antigen

SUBMITTER: Gonzalez-Ceron L 

PROVIDER: S-EPMC10058798 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature