Data Independent Acquisition Reveals In-Depth Serum Proteome Changes in Canine Leishmaniosis.

ABSTRACT: Comprehensive profiling of serum proteome provides valuable clues of health status and pathophysiological processes, making it the main strategy in biomarker discovery. However, the high dynamic range significantly decreases the number of detectable proteins, obstructing the insights into the underlying biological processes. To circumvent various serum enrichment methods, obtain high-quality proteome wide information using the next-generation proteomic, and study host response in canine leishmaniosis, we applied data-independent acquisition mass spectrometry (DIA-MS) for deep proteomic profiling of clinical samples. The non-depleted serum samples of healthy and naturally Leishmania-infected dogs were analyzed using the label-free 60-min gradient sequential window acquisition of all

SUBMITTER: Martinkovic F 

PROVIDER: S-EPMC10059658 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature