Dissecting Phenotype from Genotype with Clinical Isolates of SARS-CoV-2 First Wave Variants.

ABSTRACT: The emergence and availability of closely related clinical isolates of SARS-CoV-2 offers a unique opportunity to identify novel nonsynonymous mutations that may impact phenotype. Global sequencing efforts show that SARS-CoV-2 variants have emerged and then been replaced since the beginning of the pandemic, yet we have limited information regarding the breadth of variant-specific host responses. Using primary cell cultures and the K18-hACE2 mouse, we investigated the replication, innate immune response, and pathology of closely related, clinical variants circulating during the first wave of the pandemic. Mathematical modeling of the lung viral replication of four clinical isolates showed a dichotomy between two B.1. isolates with significantly faster and slower infected cell clearance rates

SUBMITTER: Taylor MK 

PROVIDER: S-EPMC10059853 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature