Regulation of Hypoxic-Adenosinergic Signaling by Estrogen: Implications for Microvascular Injury.

ABSTRACT: Loss of estrogen, as occurs with normal aging, leads to increased inflammation, pathologic angiogenesis, impaired mitochondrial function, and microvascular disease. While the influence of estrogens on purinergic pathways is largely unknown, extracellular adenosine, generated at high levels by CD39 and CD73, is known to be anti-inflammatory in the vasculature. To further define the cellular mechanisms necessary for vascular protection, we investigated how estrogen modulates hypoxic-adenosinergic vascular signaling responses and angiogenesis. Expression of estrogen receptors, purinergic mediators inclusive of adenosine, adenosine deaminase (ADA), and ATP were measured in human endothelial cells. Standard tube formation and wound healing assays were performed to assess angiogenesis in vitro.

SUBMITTER: Cassavaugh J 

PROVIDER: S-EPMC10059944 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature