Polymer film-based microwell array platform for long-term culture and research of human bronchial organoids.

ABSTRACT: The culture of lung organoids relies on drops of basement membrane matrices. This comes with limitations, for example, concerning the microscopic monitoring and imaging of the organoids in the drops. Also, the culture technique is not easily compatible with micromanipulations of the organoids. In this study, we investigated the feasibility of the culture of human bronchial organoids in defined x-, y- and z-positions in a polymer film-based microwell array platform. The circular microwells have thin round/U-bottoms. For this, single cells are first precultured in drops of basement membrane extract (BME). After they form cell clusters or premature organoids, the preformed structures are then transferred into the microwells in a solution of 50% BME in medium. There, the structures can be cult

SUBMITTER: Baptista D 

PROVIDER: S-EPMC10060184 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature