Gα_i-derived peptide binds the µ-opioid receptor.

ABSTRACT:

Background

G protein-coupled receptors (GPCRs) transduce external stimuli into the cell by G proteins via an allosteric mechanism. Agonist binding to the receptor stimulates GDP/GTP exchange within the heterotrimeric G protein complex, whereas recent structures of GPCR-G protein complexes revealed that the H5, S1 and S2 domains of Gα are involved in binding the active receptor, earlier studies showed that a short peptide analog derived from the C-terminus (H5) of the G protein transducin (G_t) is sufficient to stabilize rhodopsin in an active form.

Methods

We have used Molecular Dynamics simulations along with biological evaluation by means of radio-ligand binding assay to study the interactions between Gα_i-derived peptide (G-peptide) and the µ-opioid re

SUBMITTER: Kosson P 

PROVIDER: S-EPMC10060287 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature