A birefringent spectral demultiplexer enables fast hyper-spectral imaging of protoporphyrin IX during neurosurgery.

ABSTRACT: Hyperspectral imaging and spectral analysis quantifies fluorophore concentration during fluorescence-guided surgery^1-6. However, acquisition of the multiple wavelengths required to implement these methods can be time-consuming and hinder surgical workflow. To this end, a snapshot hyperspectral imaging system capable of acquiring 64 channels of spectral data simultaneously was developed for rapid hyperspectral imaging during neurosurgery. The system uses a birefringent spectral demultiplexer to split incoming light and redirect wavelengths to different sections of a large format microscope sensor. Its configuration achieves high optical throughput, accepts unpolarized input light and exceeds channel count of prior image-replicating imaging spectrometers by 4-fold. Tissue-simulati

SUBMITTER: Marois M 

PROVIDER: S-EPMC10060426 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature