The importance of unambiguous cell origin determination in neuronal repopulation studies.

ABSTRACT: Neuronal repopulation achieved through transplantation or transdifferentiation from endogenous sources holds tremendous potential for restoring function in chronic neurodegenerative disease or acute injury. Key to the evaluation of neuronal engraftment is the definitive discrimination of new or donor neurons from preexisting cells within the host tissue. Recent work has identified mechanisms by which genetically encoded donor cell reporters can be transferred to host neurons through intercellular material transfer. In addition, labeling transplanted and endogenously transdifferentiated neurons through viral vector transduction can yield misexpression in host cells in some circumstances. These issues can confound the tracking and evaluation of repopulated neurons in regenerative experimenta

SUBMITTER: Johnson TV 

PROVIDER: S-EPMC10060674 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature