Characterizing the differential distribution and targets of Sumo1 and Sumo2 in the mouse brain.

ABSTRACT: SUMOylation is an evolutionarily conserved eukaryotic posttranslational protein modification with broad biological relevance. Differentiating between the major small ubiquitin-like modifier (SUMO) paralogs and uncovering paralog-specific functions in vivo has long been very difficult. To overcome this problem, we generated His₆-HA-Sumo2 and HA-Sumo2 knockin mouse lines, expanding upon our existing His₆-HA-Sumo1 mouse line, to establish a "toolbox" for Sumo1-Sumo2 comparisons in vivo. Leveraging the specificity of the HA epitope, we performed whole-brain imaging and uncovered regional differences between Sumo1 and Sumo2 expression. At the subcellular level, Sumo2 was specifically detected in extranuclear compartments, including synapses. Immunoprecipitati

SUBMITTER: Suk TR 

PROVIDER: S-EPMC10060683 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature