Gβγ subunits colocalize with RNA polymerase II and regulate transcription in cardiac fibroblasts.

ABSTRACT: Gβγ subunits mediate many different signaling processes in various compartments of the cell, including the nucleus. To gain insight into the functions of nuclear Gβγ signaling, we investigated the functional role of Gβγ signaling in the regulation of GPCR-mediated gene expression in primary rat neonatal cardiac fibroblasts. We identified a novel, negative, regulatory role for the Gβ₁γ dimer in the fibrotic response. Depletion of Gβ₁ led to derepression of the fibrotic response at the mRNA and protein levels under basal conditions and an enhanced fibrotic response after sustained stimulation of the angiotensin II type I receptor. Our genome-wide chromatin immunoprecipitation experiments revealed that Gβ₁ colocalized and interacted with RNA polymerase II on f

SUBMITTER: Khan SM 

PROVIDER: S-EPMC10060754 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature