Effect of sirolimus on insulin dynamics in horses.

ABSTRACT:

Background

Sirolimus, a mechanistic target of rapamycin inhibitor, suppresses insulin production in other species and has therapeutic potential for hyperinsulinemia in horses.

Hypothesis/objective

Determine the pharmacokinetics (PKs) of sirolimus and evaluate its effect on insulin dynamics in healthy and insulin dysregulation (ID) horses.

Animals

Eight Standardbred geldings.

Methods

A PK study was performed followed by a placebo-controlled, randomized, crossover study. Blood sirolimus concentrations were measured by liquid chromatography-mass-spectrometry. PK indices were estimated by fitting a 2-compartment model using nonlinear least squares regression. An oral glucose test (OGT) was conducted before and 4, 24, 72, and 144 hours after administration of siroli

SUBMITTER: de Tonnerre DJ 

PROVIDER: S-EPMC10061188 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature