Rapid, simple, and effective strategy to produce monoclonal antibodies targeting protein structures using hybridoma technology.

ABSTRACT:

Background

Monoclonal antibodies are essential in life science research and developing antibody drugs and test drugs. Various methods have been developed to obtain monoclonal antibodies, among which hybridoma technology continues to be widely used. However, developing a rapid and efficient method for obtaining conformation-specific antibodies using hybridoma technology remains challenging. We previously developed the membrane-type immunoglobulin-directed hybridoma screening (MIHS) method, which is a flow cytometry-based screening technique based on the interaction between the B-cell receptor expressed on the hybridoma cell surface and the antigen protein, to obtain conformation-specific antibodies.

Results

In this study, we proposed a streptavidin-anchored ELISA screening te

SUBMITTER: Sakaguchi A 

PROVIDER: S-EPMC10061363 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature