Real-Time Library Search Increases Cross-Link Identification Depth across All Levels of Sample Complexity.

ABSTRACT: Cross-linking mass spectrometry (XL-MS) is a universal tool for probing structural dynamics and protein-protein interactions in vitro and in vivo. Although cross-linked peptides are naturally less abundant than their unlinked counterparts, recent experimental advances improved cross-link identification by enriching the cross-linker-modified peptides chemically with the use of enrichable cross-linkers. However, mono-links (i.e., peptides modified with a hydrolyzed cross-linker) still hinder efficient cross-link identification since a large proportion of measurement time is spent on their MS2 acquisition. Currently, cross-links and mono-links cannot be separated by sample preparation techniques or chromatography because they are chemically almost identical. Here, we foun

SUBMITTER: Ruwolt M 

PROVIDER: S-EPMC10061366 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature