Multidimensional Protein Solubility Optimization with an Ultrahigh-Throughput Microfluidic Platform.

ABSTRACT: Protein-based biologics are highly suitable for drug development as they exhibit low toxicity and high specificity for their targets. However, for therapeutic applications, biologics must often be formulated to elevated concentrations, making insufficient solubility a critical bottleneck in the drug development pipeline. Here, we report an ultrahigh-throughput microfluidic platform for protein solubility screening. In comparison with previous methods, this microfluidic platform can make, incubate, and measure samples in a few minutes, uses just 20 μg of protein (>10-fold improvement), and yields 10,000 data points (1000-fold improvement). This allows quantitative comparison of formulation excipients, such as sodium chloride, polysorbate, histidine, arginine, and sucrose. Additionally, we c

SUBMITTER: Erkamp NA 

PROVIDER: S-EPMC10061369 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature