Gut commensal Enterocloster species host inoviruses that are secreted in vitro and in vivo.

ABSTRACT:

Background

Bacteriophages in the family Inoviridae, or inoviruses, are under-characterized phages previously implicated in bacterial pathogenesis by contributing to biofilm formation, immune evasion, and toxin secretion. Unlike most bacteriophages, inoviruses do not lyse their host cells to release new progeny virions; rather, they encode a secretion system that actively pumps them out of the bacterial cell. To date, no inovirus associated with the human gut microbiome has been isolated or characterized.

Results

In this study, we utilized in silico, in vitro, and in vivo methods to detect inoviruses in bacterial members of the gut microbiota. By screening a representative genome library of gut commensals, we detected inovirus prophages in Enterocloster spp. (formerly Clostri

SUBMITTER: Burckhardt JC 

PROVIDER: S-EPMC10061712 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature