Evaluation of reference genes for transcript analyses in Komagataella phaffii (Pichia pastoris).

ABSTRACT:

Background

The yeast Komagataella phaffii (Pichia pastoris) is routinely used for heterologous protein expression and is suggested as a model organism for yeast. Despite its importance and application potential, no reference gene for transcript analysis via RT-qPCR assays has been evaluated to date. In this study, we searched publicly available RNASeq data for stably expressed genes to find potential reference genes for relative transcript analysis by RT-qPCR in K. phaffii. To evaluate the applicability of these genes, we used a diverse set of samples from three different strains and a broad range of cultivation conditions. The transcript levels of 9 genes were measured and compared using commonly applied bioinformatic tools.

Results

We could demonstrate that the often-used

SUBMITTER: Besleaga M 

PROVIDER: S-EPMC10061771 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature