Reprogramming anchorage dependency by adherent-to-suspension transition promotes metastatic dissemination.

ABSTRACT:

Background

Although metastasis is the foremost cause of cancer-related death, a specialized mechanism that reprograms anchorage dependency of solid tumor cells into circulating tumor cells (CTCs) during metastatic dissemination remains a critical area of challenge.

Methods

We analyzed blood cell-specific transcripts and selected key Adherent-to-Suspension Transition (AST) factors that are competent to reprogram anchorage dependency of adherent cells into suspension cells in an inducible and reversible manner. The mechanisms of AST were evaluated by a series of in vitro and in vivo assays. Paired samples of primary tumors, CTCs, and metastatic tumors were collected from breast cancer and melanoma mouse xenograft models and patients with de novo metastasis. Analyses of single-

SUBMITTER: Huh HD 

PROVIDER: S-EPMC10061822 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature