Gene expression variability in long-term survivors of childhood cancer and cancer-free controls in response to ionizing irradiation.

ABSTRACT:

Background

Differential expression analysis is usually adjusted for variation. However, most studies that examined the expression variability (EV) have used computations affected by low expression levels and did not examine healthy tissue. This study aims to calculate and characterize an unbiased EV in primary fibroblasts of childhood cancer survivors and cancer-free controls (N0) in response to ionizing radiation.

Methods

Human skin fibroblasts of 52 donors with a first primary neoplasm in childhood (N1), 52 donors with at least one second primary neoplasm (N2 +), as well as 52 N0 were obtained from the KiKme case-control study and exposed to a high (2 Gray) and a low dose (0.05 Gray) of X-rays and sham- irradiation (0 Gray). Genes were then classified as hypo-, non-, or hy

SUBMITTER: Grandt CL 

PROVIDER: S-EPMC10061869 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature