Highly Hydrophobic Films of Engineered Silk Proteins by a Simple Deposition Method.

ABSTRACT: Molecular engineering of protein structures offers a uniquely versatile route for novel functionalities in materials. Here, we describe a method to form highly hydrophobic thin films using genetically engineered spider silk proteins. We used structurally engineered protein variants containing ADF3 and AQ12 spider silk sequences. Wetting properties were studied using static and dynamic contact angle measurements. Solution conditions and the surrounding humidity during film preparation were key parameters to obtain high hydrophobicity, as shown by contact angles in excess of 120°. Although the surface layer was highly hydrophobic, its structure was disrupted by the added water droplets. Crystal-like structures were found at the spots where water droplets had been placed. To understand the me

SUBMITTER: Valisalmi T 

PROVIDER: S-EPMC10061925 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature