Fast and easy bioassay for the necrotizing fungus Botrytis cinerea on poplar leaves.

ABSTRACT:

Background

Necrotizing pathogens pose an immense economic and ecological threat to trees and forests, but the molecular analysis of these pathogens is still in its infancy because of lacking model systems. To close this gap, we developed a reliable bioassay for the widespread necrotic pathogen Botrytis cinerea on poplars (Populus sp.), which are established model organisms to study tree molecular biology.

Results

Botrytis cinerea was isolated from Populus x canescens leaves. We developed an infection system using fungal agar plugs, which are easy to handle. The method does not require costly machinery and results in very high infection success and significant fungal proliferation within four days. We successfully tested the fungal plug infection on 18 poplar species from fiv

SUBMITTER: Dreischhoff S 

PROVIDER: S-EPMC10061990 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature