Modulation of Capsid Dynamics in Bromoviruses by the Host and Heterologous Viral Replicase.

ABSTRACT: The three genomic and a single subgenomic RNA of Cowpea chlorotic mottle virus (CCMV), which is pathogenic to plants, is packaged into three morphologically indistinguishable icosahedral virions with T=3 symmetry. The two virion types, C1^V and C2^V, package genomic RNAs 1 (C1) and 2 (C2), respectively. The third virion type, C3+4^V, copackages genomic RNA3 and its subgenomic RNA (RNA4). In this study, we sought to evaluate how the alteration of native capsid dynamics by the host and viral replicase modulate the general biology of the virus. The application of a series of biochemical, molecular, and biological assays revealed the following. (i) Proteolytic analysis of the three virion types of CCMV assembled individually in planta revealed that,

SUBMITTER: Chakravarty A 

PROVIDER: S-EPMC10062129 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature