The Genetic Variation of Porcine Reproductive and Respiratory Syndrome Virus Replicase Protein nsp2 Modulates Viral Virulence and Persistence.

ABSTRACT: Fast evolution in the field of the replicase nsp2 represents a most prominent feature of porcine reproductive and respiratory syndrome virus (PRRSV). Here, we determined its biological significance in viral pathogenesis by constructing interlineage chimeric mutants between the Chinese highly pathogenic PRRSV (HP-PRRSV) strain JXwn06 (lineage 8) and the low-virulent NADC30-like strain CHsx1401 (lineage 1). Replacement with nsp2 from JXwn06 was surprisingly lethal to the backbone virus CHsx1401, but combined substitution with the structural protein-coding region (SP) gave rise to viable virus CHsx1401-SPnsp2_JX. Meanwhile, a derivative carrying only the SP region (CHsx1401-SP_JX) served as a control. Subsequent animal experiments revealed that acquisition of SP alone (CHs

SUBMITTER: Kong C 

PROVIDER: S-EPMC10062138 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature