Continuous freeze-drying of messenger RNA lipid nanoparticles enables storage at higher temperatures.

ABSTRACT: Messenger RNA (mRNA) lipid nanoparticles (LNPs) have emerged at the forefront during the COVID-19 vaccination campaign. Despite their tremendous success, mRNA vaccines currently require storage at deep freeze temperatures which complicates their storage and distribution, and ultimately leads to lower accessibility to low- and middle-income countries. To elaborate on this challenge, we investigated freeze-drying as a method to enable storage of mRNA LNPs at room- and even higher temperatures. More specifically, we explored a novel continuous freeze-drying technique based on spin-freezing, which has several advantages compared to classical batch freeze-drying including a much shorter drying time and improved process and product quality controlling. Here, we give insight into the variables th

SUBMITTER: Meulewaeter S 

PROVIDER: S-EPMC10062427 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature