Detection of specific uncultured bacteriophages by fluorescence in situ hybridisation in pig microbiome.

ABSTRACT: Microbial communities have huge impacts on their ecosystems and local environments spanning from marine and soil communities to the mammalian gut. Bacteriophages (phages) are important drivers of population control and diversity in the community, but our understanding of complex microbial communities is halted by biased detection techniques. Metagenomics have provided a method of novel phage discovery independent of in vitro culturing techniques and have revealed a large proportion of understudied phages. Here, five jumbophage genomes, that were previously assembled in silico from pig faecal metagenomes, are detected and observed directly in their natural environment using a modified phageFISH approach, and combined with methods to decrease bias against large-sized phages (e.g., jumbophage

SUBMITTER: Ostenfeld LJ 

PROVIDER: S-EPMC10062541 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature