Analysis of the external signals driving the transcriptional regulation of the main genes involved in denitrification in Haloferax mediterranei.

ABSTRACT: Haloferax mediterranei is the model microorganism for the study of the nitrogen cycle in haloarchaea. This archaeon not only assimilate N-species such as nitrate, nitrite, or ammonia, but also it can perform denitrification under low oxygen conditions, using nitrate or nitrite as alternative electron acceptors. However, the information currently available on the regulation of this alternative respiration in this kind of microorganism is scarce. Therefore, in this research, the study of haloarchaeal denitrification using H. mediterranei has been addressed by analyzing the promoter regions of the four main genes of denitrification (narGH, nirK, nor, and nosZ) through bioinformatics, reporter gene assays under oxic and anoxic conditions and by

SUBMITTER: Miralles-Robledillo JM 

PROVIDER: S-EPMC10062603 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature