Metheor: Ultrafast DNA methylation heterogeneity calculation from bisulfite read alignments.

ABSTRACT: Phased DNA methylation states within bisulfite sequencing reads are valuable source of information that can be used to estimate epigenetic diversity across cells as well as epigenomic instability in individual cells. Various measures capturing the heterogeneity of DNA methylation states have been proposed for a decade. However, in routine analyses on DNA methylation, this heterogeneity is often ignored by computing average methylation levels at CpG sites, even though such information exists in bisulfite sequencing data in the form of phased methylation states, or methylation patterns. In this study, to facilitate the application of the DNA methylation heterogeneity measures in downstream epigenomic analyses, we present a Rust-based, extremely fast and lightweight bioinformatics toolkit cal

SUBMITTER: Lee D 

PROVIDER: S-EPMC10062925 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature