Selective whole-genome amplification reveals population genetics of Leishmania braziliensis directly from patient skin biopsies.

ABSTRACT: In Brazil, Leishmania braziliensis is the main causative agent of the neglected tropical disease, cutaneous leishmaniasis (CL). CL presents on a spectrum of disease severity with a high rate of treatment failure. Yet the parasite factors that contribute to disease presentation and treatment outcome are not well understood, in part because successfully isolating and culturing parasites from patient lesions remains a major technical challenge. Here we describe the development of selective whole genome amplification (SWGA) for Leishmania and show that this method enables culture-independent analysis of parasite genomes obtained directly from primary patient skin samples, allowing us to circumvent artifacts associated with adaptation to culture. We show that SWGA can be applied to multiple Lei

SUBMITTER: Pilling OA 

PROVIDER: S-EPMC10063166 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature