Sperm physiology and in vitro fertilising ability rely on basal metabolic activity: insights from the pig model.

ABSTRACT: Whether basal metabolic activity in sperm has any influence on their fertilising capacity has not been explored. Using the pig as a model, the present study investigated the relationship of energetic metabolism with sperm quality and function (assessed through computer-assisted sperm analysis and flow cytometry), and fertility (in vitro fertilisation (IVF) outcomes). In semen samples from 16 boars, levels of metabolites related to glycolysis, ketogenesis and Krebs cycle were determined through a targeted metabolomics approach using liquid chromatography-tandem mass spectrometry. High-quality sperm are associated to greater levels of glycolysis-derived metabolites, and oocyte fertilisation and embryo development are conditioned by the sperm metabolic status. Interestingly, glycolysis appear

SUBMITTER: Mateo-Otero Y 

PROVIDER: S-EPMC10063579 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature