Characterization of mesenchymal stem cells in human fetal bone marrow by single-cell transcriptomic and functional analysis.

ABSTRACT: Bone marrow mesenchymal stromal/stem cells (MSCs) are a heterogeneous population that can self-renew and generate stroma, cartilage, fat, and bone. Although a significant progress has been made toward recognizing about the phenotypic characteristics of MSCs, the true identity and properties of MSCs in bone marrow remain unclear. Here, we report the expression landscape of human fetal BM nucleated cells (BMNCs) based on the single-cell transcriptomic analysis. Unexpectedly, while the common cell surface markers such as CD146, CD271, and PDGFRa used for isolating MSCs were not detected, LIFR⁺PDGFRB⁺ were identified to be specific markers of MSCs as the early progenitors. In vivo transplantation demonstrated that LIFR⁺PDGFRB⁺CD45^-CD31

SUBMITTER: Zhang P 

PROVIDER: S-EPMC10063684 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature