Measuring capillary flow dynamics using interlaced two-photon volumetric scanning.

ABSTRACT: Two photon microscopy and optical coherence tomography (OCT) are two standard methods for measuring flow speeds of red blood cells in microvessels, particularly in animal models. However, traditional two photon microscopy lacks the depth of field to adequately capture the full volumetric complexity of the cerebral microvasculature and OCT lacks the specificity offered by fluorescent labeling. In addition, the traditional raster scanning technique utilized in both modalities requires a balance of image frame rate and field of view, which severely limits the study of RBC velocities in the microvascular network. Here, we overcome this by using a custom two photon system with an axicon based Bessel beam to obtain volumetric images of the microvascular network with fluorescent specificity. We c

SUBMITTER: Giblin JT 

PROVIDER: S-EPMC10063827 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature