Impact of chronic alcohol exposure on conventional and regulatory murine T cell subsets.

ABSTRACT:

Introduction

Chronic alcohol use poses significant negative consequences to public health and, among its many biologic effects, is associated with significant T cell dysregulation within the adaptive immune system that has yet to be fully characterized. Novel, automated strategies for high dimensional flow cytometric analysis of the immune system are rapidly improving researchers' ability to detect and characterize rare cell types.

Methods

Using a murine model of chronic alcohol ingestion in conjunction with viSNE and CITRUS analysis tools, we performed a machine-driven, exploratory analysis comparing rare splenic subpopulations within the conventional CD4⁺, regulatory CD4⁺ and CD8⁺ T cell compartments between alcohol- and water-fed animals.<

SUBMITTER: Paterson CW 

PROVIDER: S-EPMC10063870 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature