In situ reduction of gold nanoparticles-decorated MXenes-based electrochemical sensing platform for KRAS gene detection.

ABSTRACT: In this work, gold nanoparticles@Ti₃C₂ MXenes nanocomposites with excellent properties were combined with toehold-mediated DNA strand displacement reaction to construct an electrochemical circulating tumor DNA biosensor. The gold nanoparticles were synthesized in situ on the surface of Ti₃C₂ MXenes as a reducing and stabilizing agent. The good electrical conductivity of the gold nanoparticles@Ti₃C₂ MXenes composite and the nucleic acid amplification strategy of enzyme-free toehold-mediated DNA strand displacement reaction can be used to efficiently and specifically detect the non-small cell cancer biomarker circulating tumor DNA KRAS gene. The biosensor has a linear detection range of 10 fM -10 nM and a detection limi

SUBMITTER: Yu X 

PROVIDER: S-EPMC10063977 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature