Development of a reproducible small intestinal microbiota model and its integration into the SHIME^®-system, a dynamic in vitro gut model.

ABSTRACT: The human gastrointestinal tract consists of different regions, each characterized by a distinct physiology, anatomy, and microbial community. While the colonic microbiota has received a lot of attention in recent research projects, little is known about the small intestinal microbiota and its interactions with ingested compounds, primarily due to the inaccessibility of this region in vivo. This study therefore aimed to develop and validate a dynamic, long-term simulation of the ileal microbiota using the SHIME^®-technology. Essential parameters were identified and optimized from a screening experiment testing different inoculation strategies, nutritional media, and environmental parameters over an 18-day period. Subjecting a synthetic bacterial consortium to the selected

SUBMITTER: Deyaert S 

PROVIDER: S-EPMC10063983 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature