Alpha-ketoglutarate promotes alveolar bone regeneration by modulating M2 macrophage polarization.

ABSTRACT:

Objectives

Alpha-ketoglutarate (αKG) is an essential metabolite that plays a crucial role in skeletal homeostasis. Here we aim to investigate the effect of αKG on alveolar socket healing and reveal the underlying mechanism in the view of macrophage polarization.

Methods

In a murine model pretreated with or without αKG, mandibular first molars were extracted. Mandibular tissues were harvested for microCT and histological analyses. Immunofluorescence was used to evaluate macrophage polarization during healing process. Macrophages with αKG/vehicle supplementation in vitro were proceeded to quantitative real-time PCR and flow cytometry to further elucidate the mechanism.

Results

MicroCT and histological analyses showed accelerated healing and enhanced bone regenera

SUBMITTER: Li Y 

PROVIDER: S-EPMC10064115 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature