Characterization of TEMPO-oxidized chitin nanofibers with various oxidation times and its application as an enzyme immobilization support.

ABSTRACT: Chitin nanofibers have recently received increased attention and are considered to be a promising material for a wide range of applications because of their excellent characteristics. In this study, 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO)-oxidized chitin nanofibers (CNFs) with various oxidation times were prepared and characterized. CNFs with different oxidation times were then utilized for enzyme immobilization, using chymotrypsin as a model enzyme. The effects of oxidation time on enzyme immobilization were explored. Results showed characteristics of chitin nanofibers can be controlled by adjusting oxidation time. CNFs treated with TEMPO for 360 min showed the lowest crystallinity (79.13 ± 1.43%), the shortest length (241.70 ± 74.61 nm), the largest width (12.67 ± 3.43 nm), and the

SUBMITTER: Chen R 

PROVIDER: S-EPMC10064359 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature