High fat diet and PCSK9 knockout modulates lipid profile of the liver and changes the expression of lipid homeostasis related genes.

ABSTRACT:

Background

High fat diet (HFD) increases the likelihood of dyslipidemia, which can be a serious risk factor for atherosclerosis, diabetes or hepatosteatosis. Although changes in different blood lipid levels were broadly investigated, such alterations in the liver tissue have not been studied before. The aim of the current study was to investigate the effect of HFD on hepatic triglyceride (TG), diglyceride (DG) and ceramide (CER) levels and on the expression of four key genes involved in lipid homeostasis (Pcsk9, Ldlr, Cd36 and Anxa2) in the liver. In addition, the potential role of PCSK9 in the observed changes was further investigated by using PCSK9 deficient mice.

Methods

We used two in vivo models: mice kept on HFD for 20 weeks and PCSK9^-/- mice. The amount of

SUBMITTER: Nemeth K 

PROVIDER: S-EPMC10064771 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature