Temporal application of lysyl oxidase during hierarchical collagen fiber formation differentially effects tissue mechanics.

ABSTRACT: The primary source of strength in menisci, tendons, and ligaments are hierarchical collagen fibers; however, these fibers are not regenerated after injury nor in engineered replacements, resulting in limited repair options. Collagen strength is reliant on fiber alignment, density, diameter, and crosslinking. Recently, we developed a culture system which guides cells in high-density collagen gels to develop native-like hierarchically organized collagen fibers, which match native fiber alignment and diameters by 6 weeks. However, tensile moduli plateau at 1MPa, suggesting crosslinking may be lacking. Collagen crosslinking is regulated by lysyl oxidase (LOX) which forms immature crosslinks that condense into mature trivalent crosslinks. Trivalent crosslinks are thought to be the primarily sou

SUBMITTER: Bates ME 

PROVIDER: S-EPMC10064799 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature