Stress activates pronociceptive endogenous opioid signalling in DRG neurons during chronic colitis.

ABSTRACT:

Aims and background

Psychological stress accompanies chronic inflammatory diseases such as IBD, and stress hormones can exacerbate pain signalling. In contrast, the endogenous opioid system has an important analgesic action during chronic inflammation. This study examined the interaction of these pathways.

Methods

Mouse nociceptive dorsal root ganglia (DRG) neurons were incubated with supernatants from segments of inflamed colon collected from patients with chronic UC and mice with dextran sodium sulfate (cDSS)-induced chronic colitis. Stress effects were studied by adding stress hormones (epinephrine and corticosterone) to dissociated neurons or by exposing cDSS mice to water avoidance stress. Changes in excitability of colonic DRG nociceptors were measured using patch clam

SUBMITTER: Guerrero-Alba R 

PROVIDER: S-EPMC10064864 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature