Genome-Wide Screen for Enhanced Noncanonical Amino Acid Incorporation in Yeast.

ABSTRACT: Numerous applications of noncanonical amino acids (ncAAs) in basic biology and therapeutic development require efficient protein biosynthesis using an expanded genetic code. However, achieving such incorporation at repurposed stop codons in cells is generally inefficient and limited by complex cellular processes that preserve the fidelity of protein synthesis. A more comprehensive understanding of the processes that contribute to ncAA incorporation would aid in the development of genomic engineering strategies for augmenting genetic code manipulation. In this work, we used a series of fluorescent reporters to screen a pooled Saccharomyces cerevisiae molecular barcoded yeast knockout (YKO) collection. Fluorescence-activated cell sorting enabled isolation of strains encoding single-ge

SUBMITTER: Zackin MT 

PROVIDER: S-EPMC10065164 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature