Molecular test for COVID-19 diagnosis based on a colorimetric genomagnetic assay.

ABSTRACT: The world is in a long pandemic period caused by the SARS-CoV-2 virus and massive diagnostic tests to assist efforts to control the spread of the disease and also to avoid new coronavirus variants are still needed. Herein, we propose a simple and accurate saliva-based colorimetric test for the diagnosis of COVID-19. Magnetic beads (MBs) modified with a sequence of single-strand DNA (ssDNA) complementary to the N gene of the SARS-CoV-2 RNA were developed and used for magnetic capture and separation from a complex saliva sample. A second biotinylated ssDNA sequence was applied, and the colorimetric detection was carried out by adding streptavidin-horseradish peroxidase conjugate, H₂O_2, and tetramethylbenzidine (TMB) as chromogenic substrate. The test does not require vi

SUBMITTER: Regina de Oliveira T 

PROVIDER: S-EPMC10066033 | biostudies-literature | 2023 May

REPOSITORIES: biostudies-literature