Molecular mechanism on forcible ejection of ATPase inhibitory factor 1 from mitochondrial ATP synthase.

ABSTRACT: IF₁ is a natural inhibitor protein for mitochondrial F_oF₁ ATP synthase that blocks catalysis and rotation of the F₁ by deeply inserting its N-terminal helices into F₁. A unique feature of IF₁ is condition-dependent inhibition; although IF₁ inhibits ATP hydrolysis by F₁, IF₁ inhibition is relieved under ATP synthesis conditions. To elucidate this condition-dependent inhibition mechanism, we have performed single-molecule manipulation experiments on IF₁-inhibited bovine mitochondrial F₁ (bMF₁). The results show that IF₁-inhibited F₁ is efficiently activated only when F₁ is rotated in the clockwise (ATP synthesis) direction, but n

SUBMITTER: Kobayashi R 

PROVIDER: S-EPMC10066207 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature