DDX47, MeCP2, and other functionally heterogeneous factors protect cells from harmful R loops.

ABSTRACT: Unscheduled R loops can be a source of genome instability, a hallmark of cancer cells. Although targeted proteomic approaches and cellular analysis of specific mutants have uncovered factors potentially involved in R-loop homeostasis, we report a more open screening of factors whose depletion causes R loops based on the ability of activation-induced cytidine deaminase (AID) to target R loops. Immunofluorescence analysis of γH2AX caused by small interfering RNAs (siRNAs) covering 3,205 protein-coding genes identifies 59 potential candidates, from which 13 are analyzed further and show a significant increase of R loops. Such candidates are enriched in factors involved in chromatin, transcription, and RNA biogenesis and other processes. A more focused study shows that the DDX47 helicase is an

SUBMITTER: Marchena-Cruz E 

PROVIDER: S-EPMC10066596 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature