Comprehensive optimization of urinary exfoliated tumor cells tests in bladder cancer with a promising microfluidic platform.

ABSTRACT:

Background

Enrichment of urinary exfoliated tumor cells (UETCs) is a noninvasive way of bladder cancer diagnosis, but the lack of specific capture and identification of tumor cells from the urine remains a limitation that impedes the development of liquid biopsy.

Methods

The CytoBot^® 2000, a novel circulating cell isolation and enrichment platform, was used for UETCs isolation after comprehensive optimization. The commercial cell lines of bladder cancer were used in spiking assay for cell recovery test. The flow cytometry and immunofluorescent staining assays were performed for expression validation of capture target and identification markers. The performance of optimized platform was validated by 159 clinical samples and analyzed using receiver operator characte

SUBMITTER: Gao F 

PROVIDER: S-EPMC10067033 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature