Novel evolved Yarrowia lipolytica strains for enhanced growth and lipid content under high concentrations of crude glycerol.

ABSTRACT:

Background

Yarrowia lipolytica is a well-studied oleaginous yeast known for its ability to accumulate and store intracellular lipids, while growing on diverse, non-conventional substrates. Amongst them, crude glycerol, a low-cost by-product of the biodiesel industry, appears to be an interesting option for scaling up a sustainable single-cell oil production process. Adaptive laboratory evolution (ALE) is a powerful tool to force metabolic adaptations endowing tolerance to stressful environmental conditions, generating superior phenotypes with industrial relevance.

Results

Y. lipolytica MUCL 28849 underwent ALE in a synthetic medium with increasing concentration of pure or crude glycerol as a stressing factor (9-20% v/v) for 520 generations. In one case of pure glycerol, chem

SUBMITTER: Tsirigka A 

PROVIDER: S-EPMC10067222 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature